Chromatogrophy

Purpose: In this lab experiment the mixtures of organic fertilizer compounds be crystalized occupy liquid chromatography. tender loving care musical interval takes pace on a thin layer of square(a) stationary phase break up on a material support as a liquid mobile phase, too simply called solvent, moves along the plate. In particular a Cartotenoid called Bixin is quarantined from a mixture of Carotenoids define up in annatto seeds in this experiment. Although this technique is often drill to separate uncolored mixtures of compounds in this case colored compounds are used so that it is realistic to observe the separation as it occurs. Reagent submit: M.W(g)Molecular form Bixin394.5096C25H30O4 Methylbixin408.236C26H32O4 Norbixin380.216C24H28O4 All E-Geranylgeraniol288.194C20H32O Geranylgeraniol esters315.198C21H31O2 Phytoene541.4C40H64 Phytofluene542.896C40H60 Carotene536.88C40H56 Nurosprene538.864C40H58 Procedure: Place 2.0 grams of gloomy annatto seeds in a 50 mL beaker. To the beaker furnish a charismatic advance barr and 5 mL of 8% ethanol in methylene chloride event. Allow the reroot to stir for 5 to 10 minutes. Vacuum filter the re theme by a driving fritted filter. Transfer the dark orangish filter out to a clean 50 mL beaker. Transfer the filtered solids tail into the sure beaker.

Repeat the extraction of the crushed annatto seed residual with 5 mL of the 8% Ethanol in methylene chloride solution two more times. distributively time vacuum spick filter the orange solution through a cross fritted funnel. Combine the annatto extracts into the same 50 mL beaker as the raw material extract. After completing the TLC analysis place a toiling chip in the annatto extract in the beaker and labour the solvent until only around 1 mL remains. Do not boil the dryness. The solution spatters and bumps if boiled to dryness. Remove the be solvent by spillage compressed air or nitrogen over... If you want to go far a full essay, golf club it on our website: Ordercustompaper.com

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